Molecular cloning of bovine thyrotropin-releasing hormone receptor gene.

نویسندگان

  • M Takata
  • Y Shimada
  • A Ikeda
  • K Sekikawa
چکیده

The genomic DNA encoding bovine Thyrotropin-Releasing Hormone Receptor (TRHR) was isolated from a bovine (Holstein) genomic library. Using PCR fragments of bovine candidate TRHR Transmembrane domain (TMD-III-IV) and C-terminus domain of mouse TRHR cDNA as probes, 9 x 10(5) plaques were screened to obtain several clones each containing the N-terminus or C-terminus domain. The bovine TRHR gene encoded 398 amino acids and has a long intron. The identity of the deduced amino acid sequence of bovine TRHR exceeded 88% that of mouse, rat or human. RT-PCR analysis indicated TRHR mRNA to be expressed in the pituitary and brain.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Receptor for thyrotropin-releasing hormone in plasma membranes of bovine anterior pituitary gland. Role of lipids.

The receptor for thyrotropin-releasing hormone has been located in the plasma membranes of bovine anterior pituitary gland. Treatment of membranes with phospholipase A, phospholipase C, or digitonin or addition of lysophosphatidylcholine diminishes or completely destroys the ability of membranes to bind 3H-labeled thyrotropin-releasing hormone. Phospholipase D was without effect. These reductio...

متن کامل

Solubilization and characterization of thyrotropin-releasing hormone receptors from rat brain.

The thyrotropin-releasing hormone receptor from rat brain was solubilized in a stable unbound form. The natural glycoside digitonin was the only detergent of a variety tested capable of solubilizing the active receptor. The digitonin-solubilized receptor exhibited binding kinetics for 3-[3H]methylhistidine thyrotropin-releasing hormone virtually identical to membrane preparations and responded ...

متن کامل

Distribution of thyrotropin releasing hormone receptor type 2 in rats: an immunohistochemical study.

OBJECTIVE To investigate the organ distribution of thyrotropin releasing hormone receptor (TRHR) type 2 in rats by immunohistochemical method. METHODS TRHR type 2 was identified immunohistochemically in the rat tissues using specific anti-TRHR antiserum raised in New Zealand white rabbits immunized with a conjugate of synthetic TRHR type 2 (5-23) with bovine serum albumin. Immunohistochemical...

متن کامل

Carboxyl Tail Cysteine Mutants of the Thyrotropin - releasing Hormone Receptor

209 words Introduction: 474 words Discussion: 811 words Abbreviations: TRH, thyrotropin-releasing hormone; TRH-R1, TRH receptor type 1; 7TMRs, seven transmembrane-spanning receptors; GPCRs, G-protein-coupled receptors; M1R, muscarinic receptor-1. This article has not been copyedited and formatted. The final version may differ from this version. Molecular Pharmacology Fast Forward. Published on ...

متن کامل

The Therapeutic Effect of Thyrotropin Releasing Hormone in Amyotrophic Sclerosis

Although the issue has not yet been fully resolved, there is reason to believe that TRI-I may have a trophic effect on the normal motoneuron and may transien­ tly improve the neurologic deficit in ALS. Since ALS is not a simple TRI-I deficiency disease, this improvement probably occurs via a secondary mecha -nism-possibly another neurotransmitter. It is uncertain at this time whether this effec...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • The Journal of veterinary medical science

دوره 60 1  شماره 

صفحات  -

تاریخ انتشار 1998